Journal of Current Research in Scientific Medicine

CASE REPORT
Year
: 2015  |  Volume : 1  |  Issue : 1  |  Page : 49--50

Hook effect due to parenteral Vitamin B12 supplementation in Vitamin B12 assay by electrochemiluminescence immunoassay method in a patient with severe anemia


Sunil Kumar Nanda, Lopamudra Ray, Asha Dinakaran 
 Department of Biochemistry, Pondicherry Institute of Medical Sciences, Affiliated to Pondicherry University, Puducherry, India

Correspondence Address:
Sunil Kumar Nanda
Department of Biochemistry, Pondicherry Institute of Medical Sciences, Affiliated to Pondicherry University, Puducherry
India

Prozone phenomenon or hook effect has been known to occur while performing enzyme-linked immunosorbent assay. Hook effect has been documented for analytes such as prolactin and beta-HCG. We here report a rare case of prozone phenomenon observed in Vitamin B12 estimation by electrochemiluminescence immunoassay in a patient receiving parenteral Vitamin B12 supplementation.


How to cite this article:
Nanda SK, Ray L, Dinakaran A. Hook effect due to parenteral Vitamin B12 supplementation in Vitamin B12 assay by electrochemiluminescence immunoassay method in a patient with severe anemia.J Curr Res Sci Med 2015;1:49-50


How to cite this URL:
Nanda SK, Ray L, Dinakaran A. Hook effect due to parenteral Vitamin B12 supplementation in Vitamin B12 assay by electrochemiluminescence immunoassay method in a patient with severe anemia. J Curr Res Sci Med [serial online] 2015 [cited 2021 Apr 22 ];1:49-50
Available from: https://www.jcrsmed.org/article.asp?issn=2455-3069;year=2015;volume=1;issue=1;spage=49;epage=50;aulast=Nanda;type=0