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CASE REPORT
Year : 2015  |  Volume : 1  |  Issue : 1  |  Page : 49-50

Hook effect due to parenteral Vitamin B12 supplementation in Vitamin B12 assay by electrochemiluminescence immunoassay method in a patient with severe anemia


Department of Biochemistry, Pondicherry Institute of Medical Sciences, Affiliated to Pondicherry University, Puducherry, India

Correspondence Address:
Sunil Kumar Nanda
Department of Biochemistry, Pondicherry Institute of Medical Sciences, Affiliated to Pondicherry University, Puducherry
India
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Source of Support: None, Conflict of Interest: None


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Prozone phenomenon or hook effect has been known to occur while performing enzyme-linked immunosorbent assay. Hook effect has been documented for analytes such as prolactin and beta-HCG. We here report a rare case of prozone phenomenon observed in Vitamin B12 estimation by electrochemiluminescence immunoassay in a patient receiving parenteral Vitamin B12 supplementation.


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